Autologous neu DNA vaccine can be as effective as xenogenic neu DNA vaccine by altering administration route

Cheng Fen Tu, Chi Chen Lin, Ming Chuan Chen, Tai-Ming Ko, Chiu Mei Lin, Ying Chang Wang, Ming Derg Lai*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

We examined the therapeutic efficacy of xenogenic human N′-terminal neu DNA vaccine and autologous mouse N′-terminal neu DNA vaccine on MBT-2 tumor cells in C3H mice. Intramuscular injection of xenogenic and autologous neu DNA vaccines produced comparable therapeutic efficacies. Mouse and human N′-neu DNA vaccine induced tumor infiltration of CD8 + T cells, while the human vaccine was less effective at stimulating natural killer cells. Depletion of CD8 + T cells abolished the therapeutic efficacy of both types of DNA vaccines. On the other hand, xenogenic neu DNA vaccine showed significantly better therapeutic efficacy than autologous DNA vaccine with gene gun immunization. Increased infiltration of CD8 + T cells was correlated with enhanced therapeutic efficacy in the human N′-neu group of mice. Therefore, intramuscular injection can enhance the therapeutic efficacy of autologous neu DNA vaccine.

Original languageEnglish
Pages (from-to)719-728
Number of pages10
JournalVaccine
Volume25
Issue number4
DOIs
StatePublished - 8 Jan 2007

Keywords

  • Autologous
  • Cancer
  • DNA vaccine
  • Intramuscular delivery
  • Neu
  • Xenogenic

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