Array arrangement of living cells on self-assembled-monolayer pattern chip with femtosecond laser inducing mechanical force "micro tsunami"

Atushi Kira*, Kazunori Okano, Yoichiroh Hosokawa, Koh Fnwa, Jyunpei Yuyama, Akira Naito, Hiroshi Masuhara

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We have developed a novel method for arranging living cells on a glass chip with micrometer-scale cell-adhering areas by applying femtosecod laser. To form the cell-adhesion area, the chip surface was first covalently modified with perfluoroalkyl self-assembled-monolayer (Rf-SAM) and then etched by oxygen-plasma. The Rf-surface and the etched surface were characterized using solvent contact-angle analysis. It was confirmed that the Rf-surface has low surface-free-energy as contact angle was 118.2° and 69.0° with water and hexadecane, respectively. Thus the Rf-surface has repellent characteristics for polar and nonpolar materials. Protein adsorption on the fabricated Rf-micropattern chip was evaluated using R-phycoerythrin. There was little signal derived from R-phycoerythrin on the Rf-surface indicating that Rf-SAM inhibits the protein adsorption onto the surface. When mammalian PC12 cells were cultured on the Rf-micropattern chip, the cells adhered and grew up only on the etched glass surface but not onRf-surface. Cultured cells were detached one by one from a culture substrate and transported onto a specific cell-adhering area on the Rf-micropattern chip by using femtosecond-laser-induced mechanical force that we named "micro tsunami".

Original languageEnglish
Title of host publication2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
Pages387-391
Number of pages5
DOIs
StatePublished - 1 Dec 2008
Event2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation" - Nagoya, Japan
Duration: 6 Nov 20089 Nov 2008

Publication series

Name2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008

Conference

Conference2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
CountryJapan
CityNagoya
Period6/11/089/11/08

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    Kira, A., Okano, K., Hosokawa, Y., Fnwa, K., Yuyama, J., Naito, A., & Masuhara, H. (2008). Array arrangement of living cells on self-assembled-monolayer pattern chip with femtosecond laser inducing mechanical force "micro tsunami". In 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics" (pp. 387-391). [4752483] (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008). https://doi.org/10.1109/MHS.2008.4752483