Analysis of Puerariae radix and its medicinal preparations by capillary electrophoresis

Chin Yu Wang, Hsi Ya Huang, Kuang Lung Kuo, You-Zung Hsieh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

This study presents a high-performance capillary electrophoresis (CE) method to analyze five constituents of Puerariae radix, an important crude herb used in Chinese medicine. Puerarin, daidzin, daidzein, genistein and biochanin A are the bioactive constituents of Pureriae radix. Herein, those analytes were successfully separated within 6 min using a pH 10.1 borax-NaOH buffer. The effects of pH value and concentration of the running buffer on the separation of the five analytes were also examined. The relative standard deviations of the analytes' migration times were less than 0.38% under the optimized separation conditions. Notably, the correlation coefficients of the analytes' linear calibration graphs exceeded 0.998. Moreover, the amounts of the five constituents in three different Puerariae radix samples were determined by the CE method with a relatively simple extraction procedure.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalJournal of Chromatography A
Volume802
Issue number1
DOIs
StatePublished - 3 Apr 1998

Keywords

  • Biochanin A
  • Daidzein
  • Daidzin
  • Genistein
  • Pharmaceutical analysis
  • Puerariae radix
  • Puerarin

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