Analysis of prostaglandins by micellar electrokinetic capillary chromatography

You-Zung Hsieh; Kuang Lung Kuo

doi:10.1016/S0021-9673(96)00795-9

Analysis of prostaglandins by micellar electrokinetic capillary chromatography

You-Zung Hsieh^*, Kuang Lung Kuo

^*Corresponding author for this work

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

This paper presents a rapid and reliable micellar electrokinetic capillary chromatography (MEKC) method to separate major prostaglandins and thromboxane B₂. The running buffer was modified with sodium dodecyl sulfate (SDS). The effects of the SDS concentration on the migration behavior of analytes was also examined. Moreover, the influences of electrolyte concentration and capillary temperature on the separation were investigated. In optimum conditions, seven major prostaglandins and thromboxane B₂ could be separated within 8 min. The relative standard deviations of the migration times (reproducibility) of the analytes were less than 0.82%.

Original language	English
Pages (from-to)	277-284
Number of pages	8
Journal	Journal of Chromatography A
Volume	761
Issue number	1-2
DOIs	https://doi.org/10.1016/S0021-9673(96)00795-9
State	Published - 14 Feb 1997

Keywords

Buffer composition
Prostaglandins
Thromboxanes

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author = "You-Zung Hsieh and Kuo, {Kuang Lung}",

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T1 - Analysis of prostaglandins by micellar electrokinetic capillary chromatography

AU - Hsieh, You-Zung

AU - Kuo, Kuang Lung

PY - 1997/2/14

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N2 - This paper presents a rapid and reliable micellar electrokinetic capillary chromatography (MEKC) method to separate major prostaglandins and thromboxane B2. The running buffer was modified with sodium dodecyl sulfate (SDS). The effects of the SDS concentration on the migration behavior of analytes was also examined. Moreover, the influences of electrolyte concentration and capillary temperature on the separation were investigated. In optimum conditions, seven major prostaglandins and thromboxane B2 could be separated within 8 min. The relative standard deviations of the migration times (reproducibility) of the analytes were less than 0.82%.

AB - This paper presents a rapid and reliable micellar electrokinetic capillary chromatography (MEKC) method to separate major prostaglandins and thromboxane B2. The running buffer was modified with sodium dodecyl sulfate (SDS). The effects of the SDS concentration on the migration behavior of analytes was also examined. Moreover, the influences of electrolyte concentration and capillary temperature on the separation were investigated. In optimum conditions, seven major prostaglandins and thromboxane B2 could be separated within 8 min. The relative standard deviations of the migration times (reproducibility) of the analytes were less than 0.82%.

KW - Buffer composition

KW - Prostaglandins

KW - Thromboxanes

UR - http://www.scopus.com/inward/record.url?scp=0031566974&partnerID=8YFLogxK

U2 - 10.1016/S0021-9673(96)00795-9

DO - 10.1016/S0021-9673(96)00795-9

M3 - Article

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AN - SCOPUS:0031566974

VL - 761

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EP - 284

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1-2

ER -

Analysis of prostaglandins by micellar electrokinetic capillary chromatography

Abstract

Keywords

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