Analysis of prostaglandins by micellar electrokinetic capillary chromatography

This paper presents a rapid and reliable micellar electrokinetic capillary chromatography (MEKC) method to separate major prostaglandins and thromboxane B₂. The running buffer was modified with sodium dodecyl sulfate (SDS). The effects of the SDS concentration on the migration behavior of analytes was also examined. Moreover, the influences of electrolyte concentration and capillary temperature on the separation were investigated. In optimum conditions, seven major prostaglandins and thromboxane B₂ could be separated within 8 min. The relative standard deviations of the migration times (reproducibility) of the analytes were less than 0.82%.