Analysis of prostaglandins by micellar electrokinetic capillary chromatography

You-Zung Hsieh*, Kuang Lung Kuo

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

This paper presents a rapid and reliable micellar electrokinetic capillary chromatography (MEKC) method to separate major prostaglandins and thromboxane B2. The running buffer was modified with sodium dodecyl sulfate (SDS). The effects of the SDS concentration on the migration behavior of analytes was also examined. Moreover, the influences of electrolyte concentration and capillary temperature on the separation were investigated. In optimum conditions, seven major prostaglandins and thromboxane B2 could be separated within 8 min. The relative standard deviations of the migration times (reproducibility) of the analytes were less than 0.82%.

Original languageEnglish
Pages (from-to)277-284
Number of pages8
JournalJournal of Chromatography A
Volume761
Issue number1-2
DOIs
StatePublished - 14 Feb 1997

Keywords

  • Buffer composition
  • Prostaglandins
  • Thromboxanes

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