Analysis of chitin oligosaccharides by capillary electrophoresis with laser-induced fluorescence

Chin Y. Wang, You-Zung Hsieh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

A method, using capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection for analyzing chitin oligosaccharides is described. Chitin oligosaccharides were derivatized with 9-aminopyrene-1,4,6-trisulfonate (APTS) via reductive amination at 37°C for 16 h (optimized conditions). The APTS-chitin oligosaccharides were analyzed using either an acidic citric acid-phosphate buffer or an alkaline borate buffer. The effects of buffer types, buffer pH values, and buffer concentrations on the separation were examined. The analytes were successfully separated by using a pH 4.6 citric acid-phosphate within 19 min. The APTS-derivatized chitin monosaccharide (D-glucosamine) migrated first. The analytes were also completely separated by using a pH 9.0 borate buffer within 24 min. Moreover, the specificity of enzyme digestion on chitin polysaccharides using the optimized APTS labeling procedure and the CE-LIF method was demonstrated.

Original languageEnglish
Pages (from-to)431-438
Number of pages8
JournalJournal of Chromatography A
Volume979
Issue number1-2
DOIs
StatePublished - 6 Dec 2002

Keywords

  • Aminopyrenetrisulfonate
  • Chitin
  • Derivatization, electrophoresis
  • Oligosaccharides

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