Analysis of chitin oligosaccharides by capillary electrophoresis with laser-induced fluorescence

Chin Y. Wang; You-Zung Hsieh

doi:10.1016/S0021-9673(02)01260-8

Analysis of chitin oligosaccharides by capillary electrophoresis with laser-induced fluorescence

Chin Y. Wang, You-Zung Hsieh^*

^*Corresponding author for this work

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

A method, using capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection for analyzing chitin oligosaccharides is described. Chitin oligosaccharides were derivatized with 9-aminopyrene-1,4,6-trisulfonate (APTS) via reductive amination at 37°C for 16 h (optimized conditions). The APTS-chitin oligosaccharides were analyzed using either an acidic citric acid-phosphate buffer or an alkaline borate buffer. The effects of buffer types, buffer pH values, and buffer concentrations on the separation were examined. The analytes were successfully separated by using a pH 4.6 citric acid-phosphate within 19 min. The APTS-derivatized chitin monosaccharide (D-glucosamine) migrated first. The analytes were also completely separated by using a pH 9.0 borate buffer within 24 min. Moreover, the specificity of enzyme digestion on chitin polysaccharides using the optimized APTS labeling procedure and the CE-LIF method was demonstrated.

Original language	English
Pages (from-to)	431-438
Number of pages	8
Journal	Journal of Chromatography A
Volume	979
Issue number	1-2
DOIs	https://doi.org/10.1016/S0021-9673(02)01260-8
State	Published - 6 Dec 2002

Keywords

Aminopyrenetrisulfonate
Chitin
Derivatization, electrophoresis
Oligosaccharides

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title = "Analysis of chitin oligosaccharides by capillary electrophoresis with laser-induced fluorescence",

abstract = "A method, using capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection for analyzing chitin oligosaccharides is described. Chitin oligosaccharides were derivatized with 9-aminopyrene-1,4,6-trisulfonate (APTS) via reductive amination at 37°C for 16 h (optimized conditions). The APTS-chitin oligosaccharides were analyzed using either an acidic citric acid-phosphate buffer or an alkaline borate buffer. The effects of buffer types, buffer pH values, and buffer concentrations on the separation were examined. The analytes were successfully separated by using a pH 4.6 citric acid-phosphate within 19 min. The APTS-derivatized chitin monosaccharide (D-glucosamine) migrated first. The analytes were also completely separated by using a pH 9.0 borate buffer within 24 min. Moreover, the specificity of enzyme digestion on chitin polysaccharides using the optimized APTS labeling procedure and the CE-LIF method was demonstrated.",

keywords = "Aminopyrenetrisulfonate, Chitin, Derivatization, electrophoresis, Oligosaccharides",

author = "Wang, {Chin Y.} and You-Zung Hsieh",

year = "2002",

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doi = "10.1016/S0021-9673(02)01260-8",

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pages = "431--438",

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TY - JOUR

T1 - Analysis of chitin oligosaccharides by capillary electrophoresis with laser-induced fluorescence

AU - Wang, Chin Y.

AU - Hsieh, You-Zung

PY - 2002/12/6

Y1 - 2002/12/6

N2 - A method, using capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection for analyzing chitin oligosaccharides is described. Chitin oligosaccharides were derivatized with 9-aminopyrene-1,4,6-trisulfonate (APTS) via reductive amination at 37°C for 16 h (optimized conditions). The APTS-chitin oligosaccharides were analyzed using either an acidic citric acid-phosphate buffer or an alkaline borate buffer. The effects of buffer types, buffer pH values, and buffer concentrations on the separation were examined. The analytes were successfully separated by using a pH 4.6 citric acid-phosphate within 19 min. The APTS-derivatized chitin monosaccharide (D-glucosamine) migrated first. The analytes were also completely separated by using a pH 9.0 borate buffer within 24 min. Moreover, the specificity of enzyme digestion on chitin polysaccharides using the optimized APTS labeling procedure and the CE-LIF method was demonstrated.

AB - A method, using capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection for analyzing chitin oligosaccharides is described. Chitin oligosaccharides were derivatized with 9-aminopyrene-1,4,6-trisulfonate (APTS) via reductive amination at 37°C for 16 h (optimized conditions). The APTS-chitin oligosaccharides were analyzed using either an acidic citric acid-phosphate buffer or an alkaline borate buffer. The effects of buffer types, buffer pH values, and buffer concentrations on the separation were examined. The analytes were successfully separated by using a pH 4.6 citric acid-phosphate within 19 min. The APTS-derivatized chitin monosaccharide (D-glucosamine) migrated first. The analytes were also completely separated by using a pH 9.0 borate buffer within 24 min. Moreover, the specificity of enzyme digestion on chitin polysaccharides using the optimized APTS labeling procedure and the CE-LIF method was demonstrated.

KW - Aminopyrenetrisulfonate

KW - Chitin

KW - Derivatization, electrophoresis

KW - Oligosaccharides

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