An investigation into the influence of secondary structures on DNA hybridization using surface plasmon resonance biosensing

Fan Ching Chien, Jih Shiou Liu, Huang Ju Su, Li An Kao, Chung Fan Chiou, Wen Yih Chen, Shean-Jen Chen*

*Corresponding author for this work

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

This study utilizes a surface plasmon resonance (SPR) biosensing and a theoretical secondary structure calculation to investigate the influence of secondary structures on the DNA hybridization. It is found that the SPR angular shifts associated with the three pairs of 60mer oligonucleotides with prominent secondary structures are lower than those observed for the two pairs of oligonucleotides with no obvious secondary structures. It is also determined that increasing the DNA hybridization temperature from 35 to 45 °C reduces secondary structure effects. On the hybridization with mixture target oligonucleotides, the SPR results demonstrate that secondary structures interfere significantly.

Original languageEnglish
Pages (from-to)429-434
Number of pages6
JournalChemical Physics Letters
Volume397
Issue number4-6
DOIs
StatePublished - 21 Oct 2004

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