Absorption effects on total-internal-reflection fluorescence spectroscopy

Minoru Toriumi, Masatoshi Yanagimachi, Hiroshi Masuhara*

*Corresponding author for this work

Research output: Contribution to journalArticle

20 Scopus citations


A theoretical consideration is made for transverse-electric polarization vectors of a transmitted field in an optically absorbing sample to derive a fluorescence intensity formula as a function of the absorption indexof the sample as well as incident and observation angles in total-internal-reflection fluorescence spectroscopy. These equations are experimentally confirmed. It is shown that the angular spectrum of fluorescence intensity is the Laplace transformation of the effective penetration depth, even for the absorbing sample. High resolution of the depth profile is expected for an absorbing sample compared with a nonabsorbing one.

Original languageEnglish
Pages (from-to)6376-6382
Number of pages7
JournalApplied Optics
Issue number30
StatePublished - 20 Oct 1992

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