A unique and potent protein binding nature of liposome containing polyethylenimine and polyethylene Glycol: A nondisplaceable property

Yen Ku Liu, Yu Ling Lin, Chia Hung Chen, Ching Min Lin, Kuan Lun Ma, Fu Hsuan Chou, Jin Sheng Tsai, Hsin Yu Lin, Fu Rong Chen, Tian Lu Cheng, Chia-Ching Chang, Kuang-Wen Liao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Most of the currently available targeting vectors are produced via the linkage of targeting molecules. However, the coupling process is complicated, and the covalent linkage may attenuate the activity of certain targeting molecules. In this study, we have developed a cationic liposome complexed with polyethylenimine and polyethylene glycol polymers (LPPC) that can capture various proteins without covalent conjugation. Characterizations of prepared LPPC revealed that the maximal-binding capacity was about 170μg of bovine serum albumin to 40μg of sphere-shaped LPPC (180nm). The proteins were essentially located at or near the surface when analyzed by atomic force or transmission electron microscopy. We demonstrate that polyethylenimine was an essential component to bind the proteins. Upon the saturation of captured proteins, a given protein could not be displaced by other additional proteins and still retained its biological activity. Using a variety of functional proteins, we show some typical examples of the utility of incorporated beta-glucuronidase and antibodies onto the LPPC. The beta-glucuronidase can be used for the study of antigen-antibody interactions, whereas in studies with the antibody complex, we used anti-CD3 as an agonist to stimulate the proliferation of peripheral blood mononuclear cells via a receptor-mediated mechanism and anti-VEGFR for cell staining. In conclusion, the prepared LPPC can provide a platform to capture biologically and biochemically functional proteins on its surface for various applications, such as cell signaling, cell profiling, noncovalent enzyme-linked immunoassays, and others not mentioned.

Original languageEnglish
Pages (from-to)1318-1327
Number of pages10
JournalBiotechnology and Bioengineering
Volume108
Issue number6
DOIs
StatePublished - 1 Jun 2011

Keywords

  • Cell staining
  • Lipid coating
  • Liposomes
  • Polyethylenimine
  • Protein capturing

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