A "turn on/off'' biosensor for diagnosis of exon 7 of the SMN1 gene was developed by employing a "scorpion primer''. This scorpion primer was based on the principle of fluorescence resonance energy transfer using a fluorophore, a blocker and a quencher. It was successfully applied to detect 10 volunteer samples, and not only to in vitro testing.
- POLYMERASE-CHAIN-REACTION; CAPILLARY-ELECTROPHORESIS; MOLECULAR BEACONS; DNA-POLYMERASE; PCR; AMPLIFICATION; QUANTIFICATION; IDENTIFICATION; TEMPERATURE; AMPLICONS
Chen, C-A., Wang, C. C., Chen, Y-L., Jong, Y-J., & Wu, S-M. (2014). A "turn on/off" scorpion biosensor targeting point mutation of SMN genes for diagnosis of spinal muscular atrophy. Chemical Communications, 50(59), 8069-8072. https://doi.org/10.1039/c4cc03472d