A "turn on/off" scorpion biosensor targeting point mutation of SMN genes for diagnosis of spinal muscular atrophy

Chung-An Chen, Chun Chi Wang, Yen-Ling Chen, Yuh-Jyh Jong, Shou-Mei Wu

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A "turn on/off'' biosensor for diagnosis of exon 7 of the SMN1 gene was developed by employing a "scorpion primer''. This scorpion primer was based on the principle of fluorescence resonance energy transfer using a fluorophore, a blocker and a quencher. It was successfully applied to detect 10 volunteer samples, and not only to in vitro testing.
Original languageEnglish
Pages (from-to)8069-8072
Number of pages4
JournalChemical Communications
Volume50
Issue number59
DOIs
StatePublished - 2014

Keywords

  • POLYMERASE-CHAIN-REACTION; CAPILLARY-ELECTROPHORESIS; MOLECULAR BEACONS; DNA-POLYMERASE; PCR; AMPLIFICATION; QUANTIFICATION; IDENTIFICATION; TEMPERATURE; AMPLICONS

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