A turn-on fluorescent sensor for cysteine based on BODIPY functionalized Au nanoparticles and its application in living cell imaging

Shu Hua Lo, Ming Chun Wu, Shu-Pao Wu*

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

BODIPY functionalized Au nanoparticles (PyBDP-AuNPs) have been developed for detecting cysteine. Gold nanoparticles were prepared by reducing HAuCl4 with sodium citrate, using PyBDP as the capping agent. Upon addition of cysteine, PyBDP molecules are liberated from the AuNP surface through ligand-exchange displacement based on the strong affinity of Au with the thiol group and result in green fluorescence from PyBDP molecules. This cysteine-induced displacement of PyBDP-AuNPs was monitored by fluorescent spectroscopy with a detection limit of 1.2 μM. Optimal detection of cysteine was achieved over a pH range from 5 to 12. In addition, PyBDP-AuNPs can be used as a fluorescent probe for detecting cysteine in living cells.

Original languageEnglish
Pages (from-to)1366-1371
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume221
DOIs
StatePublished - 24 Aug 2015

Keywords

  • Cysteine
  • Fluorescence
  • Gold nanoparticles
  • Imaging agents
  • Sensors

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