A turn-on fluorescent probe for hypochlorous acid based on HOCl-promoted removal of the C = N bond in BODIPY-hydrazone

Wei Chieh Chen, Parthiban Venkatesan, Shu-Pao Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

A boron dipyrromethene-based fluorescent probe, BODH, has been successfully developed for hypochlorous acid detection based on the HOCl-promoted oxidative removal of the C = N bond in response to the amount of HOCl. The reaction is accompanied by a 6-fold increase in fluorescent quantum yield (from 0.05 to 0.29). The fluorescence intensity of the reaction between HOCl and BODH shows a good linearity in the HOCl concentration range of 1-25 μM with a low detection limit of 205 nM (S/N = 3). Confocal fluorescence microscopy imaging using RAW264.7 cells shows that the new probe, BODH, can be used as an effective fluorescent probe for the detection of HOCl in living cells.

Original languageEnglish
Pages (from-to)6892-6898
Number of pages7
JournalNew Journal of Chemistry
Volume39
Issue number9
DOIs
StatePublished - 26 Jun 2015

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