A target-specific approach for the identification of tyrosine-sulfated hemostatic proteins

Tzu An Liu, Shin Yasuda, Frederick E. Williams, Ming Yih Liu, Masahito Suiko, Yoichi Sakakibara, Yuh-Shyong Yang, Ming Cheh Liu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A simple methodology for the identification of hemostatic proteins that are subjected to posttranslational tyrosine sulfation was developed. The procedure involves sequence analysis of members of the three hemostatic pathways using the Sulfinator prediction algorithm, followed by [35S]sulfate labeling of cultured HepG2 human hepatoma cells, immunoprecipitation of targeted [35S]sulfate-labeled hemostatic proteins, and tyrosine O-[35S]sulfate analysis of immunoprecipitated proteins. Three new tyrosine-sulfated hemostatic proteins-protein S, prekallikrein, and plasminogen-were identified. Such a target-specific approach will allow investigation of tyrosine-sulfated proteins of other biochemical/physiological pathways/processes and contribute to a better understanding of the functional role of posttranslational tyrosine sulfation.

Original languageEnglish
Pages (from-to)88-90
Number of pages3
JournalAnalytical Biochemistry
Volume390
Issue number1
DOIs
StatePublished - 1 Jul 2009

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