TY - JOUR
T1 - A target-specific approach for the identification of tyrosine-sulfated hemostatic proteins
AU - Liu, Tzu An
AU - Yasuda, Shin
AU - Williams, Frederick E.
AU - Liu, Ming Yih
AU - Suiko, Masahito
AU - Sakakibara, Yoichi
AU - Yang, Yuh-Shyong
AU - Liu, Ming Cheh
PY - 2009/7/1
Y1 - 2009/7/1
N2 - A simple methodology for the identification of hemostatic proteins that are subjected to posttranslational tyrosine sulfation was developed. The procedure involves sequence analysis of members of the three hemostatic pathways using the Sulfinator prediction algorithm, followed by [35S]sulfate labeling of cultured HepG2 human hepatoma cells, immunoprecipitation of targeted [35S]sulfate-labeled hemostatic proteins, and tyrosine O-[35S]sulfate analysis of immunoprecipitated proteins. Three new tyrosine-sulfated hemostatic proteins-protein S, prekallikrein, and plasminogen-were identified. Such a target-specific approach will allow investigation of tyrosine-sulfated proteins of other biochemical/physiological pathways/processes and contribute to a better understanding of the functional role of posttranslational tyrosine sulfation.
AB - A simple methodology for the identification of hemostatic proteins that are subjected to posttranslational tyrosine sulfation was developed. The procedure involves sequence analysis of members of the three hemostatic pathways using the Sulfinator prediction algorithm, followed by [35S]sulfate labeling of cultured HepG2 human hepatoma cells, immunoprecipitation of targeted [35S]sulfate-labeled hemostatic proteins, and tyrosine O-[35S]sulfate analysis of immunoprecipitated proteins. Three new tyrosine-sulfated hemostatic proteins-protein S, prekallikrein, and plasminogen-were identified. Such a target-specific approach will allow investigation of tyrosine-sulfated proteins of other biochemical/physiological pathways/processes and contribute to a better understanding of the functional role of posttranslational tyrosine sulfation.
UR - http://www.scopus.com/inward/record.url?scp=67349284315&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2009.04.002
DO - 10.1016/j.ab.2009.04.002
M3 - Article
C2 - 19351526
AN - SCOPUS:67349284315
VL - 390
SP - 88
EP - 90
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -