A simple pumpfree HEPG2 liver cells spheroid amalgamation platform via gravity driving force

Shih Mo Yang, Chun Yen Lin, Shilpa Sivashankar, Srinivasu Valagerahally Puttaswamy, Hwan You Chang, Long Hsu, Cheng Hsien Liu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a simple pumpless microfluidics design to pair 3D artificial liver tissue. This design concept could pair two or more 3D spheroids with green approach for investigating artificial tissue cell-cell interaction. All the operation process, HepG2 cells spheroid formation and pairing, is accomplished on this chip within two days. The gravity force is applied to drive the 250μm diameter liver spheroid moving and no external power is required. Avoiding the hydrodynamic pressure damage for the assembled HepG2 cells caused by syringe pump is its unique advantage. This mimetic liver labchip is developed to hepatotoxicity assay with 3D liver tissue in the future.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1017-1019
Number of pages3
StatePublished - 1 Dec 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2 Oct 20116 Oct 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period2/10/116/10/11

Keywords

  • Liver Tissue
  • Microfluidics
  • Spheroid
  • Tissue Engineering

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    Yang, S. M., Lin, C. Y., Sivashankar, S., Puttaswamy, S. V., Chang, H. Y., Hsu, L., & Liu, C. H. (2011). A simple pumpfree HEPG2 liver cells spheroid amalgamation platform via gravity driving force. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1017-1019). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).