Traceability is important in the food supply chain to ensure the consumers' food safety, especially for the fresh products. In recent years, many solutions which applied various emerging technology have been proposed to improve the traceability of fresh product. However, the traceability system needs to be customized to satisfy different requirements. The system depends on the different product properties and supply chain models. This paper proposed a RFID-enabled traceability system for live fish supply chain. The system architecture is designed according to the specific requirement gathered in the life fish processing. Likewise, it is adaptive for the small and medium enterprises. The RFID tag is put on each live fish and is regarded as the mediator which links the live fish logistic center, retail restaurants and consumers for identification. The sensors controlled by the PLC are used to collect the information in farming as well as the automatic transporting processes. The traceability information is designed to be exchanged and used on a web-based system for farmers and consumers. The system was implemented and deployed in the live fish logistic center for trial, and the results are valuable for practical reference.