Abstract
A rapid mixing-photocrosslinking technique has been developed to investigate the kinetics of protein-nucleic acid interactions. With this technique, binding of nucleic acid to protein is first synchronized by rapid mixing in a stopped-flow apparatus. The intermediates formed at different stages of the binding process are then "frozen" by photocrosslinking with a 10-μs uv light pulse at various times after mixing. By analyzing structural changes of these intermediates as a function of time, one can obtain the information concerning the dynamic aspects of the interaction. This technique may also be applied to other macromolecular interactions in biological systems.
| Original language | English |
|---|---|
| Pages (from-to) | 481-489 |
| Number of pages | 9 |
| Journal | Analytical Biochemistry |
| Volume | 128 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1 Feb 1983 |
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Wu Lee, C-W., Hillel, Z., & Park, C. S. (1983). A rapid mixing-photocrosslinking technique to study the dynamics of nucleic acid-protein interactions. Analytical Biochemistry, 128(2), 481-489. https://doi.org/10.1016/0003-2697(83)90407-4