A rapid mixing-photocrosslinking technique to study the dynamics of nucleic acid-protein interactions

Cheng-Wen Wu Lee*, Zaharia Hillel, Chan Suk Park

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A rapid mixing-photocrosslinking technique has been developed to investigate the kinetics of protein-nucleic acid interactions. With this technique, binding of nucleic acid to protein is first synchronized by rapid mixing in a stopped-flow apparatus. The intermediates formed at different stages of the binding process are then "frozen" by photocrosslinking with a 10-μs uv light pulse at various times after mixing. By analyzing structural changes of these intermediates as a function of time, one can obtain the information concerning the dynamic aspects of the interaction. This technique may also be applied to other macromolecular interactions in biological systems.

Original languageEnglish
Pages (from-to)481-489
Number of pages9
JournalAnalytical Biochemistry
Volume128
Issue number2
DOIs
StatePublished - 1 Feb 1983

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