A new water-soluble fluorescent Cu(II) chemosensor based on tetrapeptide histidyl-glycyl-glycyl-glycine (HGGG)

Shu-Pao Wu*, Shi Rong Liu

*Corresponding author for this work

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

A new water-soluble fluorescent chemosensor Fluor-HGGG was synthesized by linking a tetrapeptide histidyl-glycyl-glycyl-glycine (HGGG) with fluorescein via standard solid-phase 9-fluorenylmethoxycarbonyl (Fmoc) chemistry. Significant fluorescence quenching was observed with Fluor-HGGG in the presence of Cu2+. Other metal ions including Ag+, Ca2+, Cd2+, Co2+, Fe2+, Fe3+, Hg2+, Mn2+, Ni2+ and Zn2+ produced only minor changes in fluorescence values for the system. The dissociation constant (KD) of Cu2+ binding in Fluor-HGGG was found to be 37 μM. The maximum fluorescence quenching caused by Cu2+ binding in Fluor-HGGG was observed over the pH range 7-7.5.

Original languageEnglish
Pages (from-to)187-191
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume141
Issue number1
DOIs
StatePublished - 18 Aug 2009

Keywords

  • Cu(II) ion
  • Fluorescein
  • Fluorescent chemosensor
  • HGGG

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