A new dimensionless index for evaluating cell stiffness-based deformability in microchannel

Chia-Hung Tsai*, Shinya Sakuma, Fumihito Arai, Makoto Kaneko

*Corresponding author for this work

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

This paper proposes a new index for evaluating the stiffness-based deformability of a cell using a microchannel. In conventional approaches, the transit time of a cell through a microchannel is often utilized for the evaluation of cell deformability. However, such time includes both the information of cell stiffness and viscosity. In this paper, we eliminate the effect from cell viscosity, and focus on the cell stiffness only. We find that the velocity of a cell varies when it enters a channel, and eventually reaches to equilibrium where the velocity becomes constant. The constant velocity is defined as the equilibrium velocity of the cell, and it is utilized to define the observability of stiffness-based deformability. The necessary and sufficient numbers of sensing points for evaluating stiffness-based deformability are discussed. Through the dimensional analysis on the microchannel system, three dimensionless parameters determining stiffness-based deformability are derived, and a new index is introduced based on these parameters. The experimental study is conducted on the red blood cells from a healthy subject and a diabetes patient. With the proposed index, we showed that the experimental data can be nicely arranged.

Original languageEnglish
Article number6697847
Pages (from-to)1187-1195
Number of pages9
JournalIEEE Transactions on Biomedical Engineering
Volume61
Issue number4
DOIs
StatePublished - 1 Jan 2014

Keywords

  • Deformability
  • microchannel
  • observability

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