A new tube cross-flow bundle heat exchanger has been designed and tested for thermophoretic deposition of submicron aerosol particles. The present design has five columns of hot and cold square tubes, respectively, arranged in a staggered manner to maintain a nearly constant temperature gradient in the direction of the aerosol flow. Each column has four tubes of 4 mm 4 mm in cross section and the gap between the tube surfaces is 0.5 mm. The precipitator was tested experimentally using monodisperse NaCl test particles ranging from 38 to 397 nm in diameter at the aerosol flow rate of 0.6 and 1.2 L/min, respectively, at different temperature gradients. Results showed that the thermophoretic deposition efficiency increased with decreasing aerosol flow rate and increasing temperature gradient with the maximum thermophoretic deposition efficiency occurred at the aerosol flow rate of 0.6 L/min. The effect of inlet temperature of the aerosol flow on the efficiency was also tested and showed increasing inlet temperature increased the deposition efficiency. Numerical simulation was further conducted to validate the experimental data and good agreement was obtained. An empirical equation was also validated to facilitate the design and scale-up of the precipitator.