A miniaturized optically-induced fluorescence-activated cell sorter

S. M. Yang*, M. Y. Ku, T. H. Punde, R. J. Chen, S. Y. Tseng, T. M. Yu, H. P. Huang, M. H. Liu, Long Hsu, C. H. Liu

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

We integrate the concept of liquid light-driven approach and a cell fluorescent detection system to sort different labeled color HepG2 and 3T3 cells. The optoelectroosmosis flow (OEOF) method would drive the liquid and reverse the flow direction immediately only with light pattern movement. A dielectrophoresis (DEP) force is applied to focal the moving cells as a line. A LabVIEW program would detect the fluorescent cell color and switch the projected light pattern automatically to manipulate the mobile cell real-time. After the OEOF operation, the selected cell is able to push a lateral displacement with 120m distance against original direction. Based on these four features, this article demonstrates a potential for cells sorting with light driving approaches.

Original languageEnglish
Title of host publication2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages326-329
Number of pages4
DOIs
StatePublished - 1 Sep 2011
Event2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China
Duration: 5 Jun 20119 Jun 2011

Publication series

Name2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

Conference

Conference2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
CountryChina
CityBeijing
Period5/06/119/06/11

Keywords

  • cell sorting
  • dielectrophoresis
  • Light-induced optoelectroosmosis flow
  • photoconductivity
  • TiOPc

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