A hypochlorous acid turn-on fluorescent probe based on HOCl-promoted oxime oxidation and its application in cell imaging

Shiang Yi Yu, Chen Yi Hsu, Wei Chieh Chen, Ling Fang Wei, Shu-Pao Wu*

*Corresponding author for this work

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

A coumarin-based fluorometric probe, HS1, has been successfully developed for highly sensitive and selective detection of hypochlorous acid (HOCl). The probe is based on the specific HOCl-promoted oxidation of oxime. The reaction is accompanied by a 7-fold increase in the fluorescent quantum yield (from 0.03 to 0.21). The fluorescence intensity of the reaction between HOCl and HS1 shows a good linearity in the HOCl concentration range of 1-20 μM and a low detection limit of 25 nM (S/N = 3). In addition, confocal fluorescence microscopy imaging using RAW264.7 macrophages demonstrates that the HS1 probe could be an efficient fluorescent probe for HOCl in living cells.

Original languageEnglish
Pages (from-to)203-207
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume196
DOIs
StatePublished - 1 Jun 2014

Keywords

  • Bioimaging
  • Coumarin
  • Fluorescent probe
  • Hypochlorous acid

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