A high-sensitivity CMOS-compatible immunosensor based on the HRP/TMB/streptavidin system

Yu Ting Tai, Yu Wei Chang, Ming Yu Lin, Yuh-Shyong Yang, Yang Tung Huang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this report, a CMOS-compatible colorimetric biosensor based on HRP/TMB/streptavidin system is proposed. HRP activity is used for labelled target quantification by photometric detector. The modulation of light intensity due to enzyme reaction can easily be detected in 96-well microarray plate by providing 650nm light source (LED). There is good correlation between absorbance in 650nm and the concentration of HRP-streptavidin with detection limit in 1.33 pM. This device is reusable and suits for various targets by microarray plate modification rather than biochip. With the advantage of portable, low cost, and reusable, this new designed immunosensor could be widely used for both research and diagnostic purposes.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Conference on Sensing Technology, ICST 2008
Pages297-300
Number of pages4
DOIs
StatePublished - 1 Dec 2008
Event3rd International Conference on Sensing Technology, ICST 2008 - Tainan, Taiwan
Duration: 30 Nov 20083 Dec 2008

Publication series

NameProceedings of the 3rd International Conference on Sensing Technology, ICST 2008

Conference

Conference3rd International Conference on Sensing Technology, ICST 2008
CountryTaiwan
CityTainan
Period30/11/083/12/08

Keywords

  • Cmos integrated circuits
  • Horseradish peroxidase
  • Immunosensor

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