A fusion protein composed of receptor binding domain of vascular endothelial growth factor-A and constant region fragment of antibody: Angiogenesis antagonistic activity

Feng Jen Tseng, Yen Ku Liu, Yo Shong Chung, Yu Ling Lin, Chia Hung Chen, Wan Yi Wang, Yu Cheng Chen, Nu Man Tsai, Tian Lu Cheng, Ru Yu Pan, Tsung Ming Hu, Ru Ping Lee, Kuang-Wen Liao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Vascular endothelial growth factor (VEGF) promotes the growth of solid tumor mainly via VEGF receptor-1 and receptor-2, which are expressed preferentially in proliferating endothelial cells. Therefore, a strategy for simultaneous blockage of both VEGF receptors may have a useful therapeutic effect in tumor growth. In this study, we utilized a fusion protein which is composed of receptor binding domain of VEGF-A (RBDV) and the constant region fragment (Fc) of a human immunoglobulin G1 (IgG1), to interfere with the growth of human umbilical vein endothelial cells (HUVECs) via VEGF receptors. The results showed that RBDV-IgG1 Fc was able to bind with both VEGF receptor-1 and receptor-2. In addition, RBDV-IgG1 Fc could decrease VEGF-induced proliferation and tube formation among HUVECs. Moreover, the cytotoxic test showed RBDV-IgG1 Fc could also enhance the cytotoxic activity of human natural killing cells. The data are suggesting that the fusion protein, RBDV-IgG1 Fc, may have potential as an angiogenesis antagonist for future tumor therapy.

Original languageEnglish
Pages (from-to)285-293
Number of pages9
JournalCytotechnology
Volume63
Issue number3
DOIs
StatePublished - 1 May 2011

Keywords

  • Fusion protein
  • Human umbilical vein endothelial cells
  • Immunoglobulin
  • Receptor binding domain of VEGF-A
  • Vascular endothelial growth factor

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