A full-field heterodyne surface plasmon resonance dynamic bio-imaging system

C. H. Lin*, Y. D. Su, C. H. Huang, K. C. Cho, Shean-Jen Chen

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Two-dimensional (2D) phase imaging system based on phase-shift interferometry (PSI) techniques can achieve a very high accuracy, but it has a degraded dynamic characteristic due to the inherent limitation of the PSI. Hence, the phase imaging system is incapable of obtaining real-time information pertaining to phase variations. To develop a label-free, high sensitivity, and dynamic bio-imaging system, a surface plasmon resonance (SPR) biosensing is combined with full-field heterodyne interferometry to develop a common-path full-field heterodyne SPR dynamic phase imager. The phase imager provides some advantages for biosensing such as label-free sensing, high sensitivity, high throughput, long-term stability, and dynamic capability. We build a 16×16 pixel photodiode array with a frame rate of up to 10 kHz as the 2D detector as opposed to a CCD camera with 30 Hz and employ an electro-optic modulator to generate a heterodyne light source. The multi-channel and real-time demodulation is calculated by utilizing a home-made digital signal processing-based lock-in amplifier. The SPR phase imager can detect refractive index changes better than 10-6 by testing the difference between nitrogen and argon gases, and will be used to analyze the biomolecular interaction on sensing surface with high throughput screening.

Original languageEnglish
Title of host publicationNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
DOIs
StatePublished - 30 Apr 2007
EventNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV - San Jose, CA, United States
Duration: 23 Jan 200724 Jan 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6447
ISSN (Print)1605-7422

Conference

ConferenceNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
CountryUnited States
CitySan Jose, CA
Period23/01/0724/01/07

Keywords

  • Dynamic bio-imaging
  • Full-field
  • Heterodyne interferometry
  • Surface plasmon resonance

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  • Cite this

    Lin, C. H., Su, Y. D., Huang, C. H., Cho, K. C., & Chen, S-J. (2007). A full-field heterodyne surface plasmon resonance dynamic bio-imaging system. In Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV [644709] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6447). https://doi.org/10.1117/12.701749