A fluorescent turn-on probe for detection of hypochlorus acid and its bioimaging in living cells

Chien Ju Chu, Guan Syuan Wu, Ho I. Ma, Parthiban Venkatesan, Natesan Thirumalaivasan, Shu Pao Wu*

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Hypochlorous acid has played several functions in the biological system. However, excess HOCl can cause damage to biomolecules and result in some diseases. Accordingly, a new fluorescent probe, BSP, has been developed for fast recognition of HOCl through the HOCl-induced oxidation of methyl phenyl sulfide to sulfoxide. The reaction of BSP with HOCl caused a 22-fold fluorescence enhancement (quantum yield increase from 0.006 to 0.133). The detection limit of HOCl is found to be 30 nM (S/N = 3). The fluorescence enhancement is due to the suppression of the photo-induced electron transfer from the methyl phenyl sulfide moiety to BODIPY. Eventually, the cellular fluorescence imaging experiment showed that BSP could be effectively used for monitoring HOCl in living cells.

Original languageEnglish
Article number118234
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume233
DOIs
StatePublished - 5 Jun 2020

Keywords

  • BODIPY
  • Cell imaging
  • Fluorescent probe
  • HOCl

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