A 975-mW Fully Integrated Genetic Variant Discovery System-on-Chip in 28 nm for Next-Generation Sequencing

Yi Chung Wu, Yen Lung Chen, Chung Hsuan Yang, Chao Hsi Lee, Chao Yang Yu, Nian Shyang Chang, Ling Chien Chen, Jia Rong Chang, Chun Pin Lin, Hung Lieh Chen, Chi Shi Chen, Jui Hung Hung*, Chia Hsiang Yang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This article presents the first dedicated system-on-chip (SoC) that supports full data analysis workflow for genetic variant discovery for next-generation sequencing (NGS). The SoC implements four major steps: preprocessing, short-read mapping, haplotype calling, and variant calling. By adopting the sBWT-based short read mapping and Genome Analysis ToolKit haplotype caller-based variant calling algorithms, this work achieves a comparable precision as the software solutions. The dedicated hardware architecture achieves high parallelism with low hardware complexity. In this SoC, the multitask sorting engine and the dynamic programming processing engine are proposed for essential computing tasks for NGS data analysis. An ARC processor is integrated to facilitate IO interfaces, including a DDR3 PHY. Fabricated in a 28-nm technology, the chip area is 12 mm2. It dissipates 975 mW at a clock frequency of 400 MHz from a 0.9-V supply. The SoC is able to complete variant discovery for the whole human genome within 37 min. Compared with an optimized graphic processing unit solution, this work is 66 × faster and also achieves an 1.59× 104 times higher energy efficiency and 3086 × higher area efficiency. Verified with the FDA standardized benchmark, the SoC achieves a precision of 99.6%. The prototype system demonstrates the analysis procedure in real time for on-site DNA sequencing.

Original languageEnglish
Article number9239376
Pages (from-to)123-135
Number of pages13
JournalIEEE Journal of Solid-State Circuits
Volume56
Issue number1
DOIs
StatePublished - Jan 2021

Keywords

  • Digital CMOS integrated circuits
  • haplotype calling
  • next-generation sequencing (NGS)
  • short-read mapping
  • system-on-chip (SoC)
  • variant calling

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