532-nm-excited hyper-Raman spectroscopy of amino acids

Chun- Wen, Chi-Nan Yu, Natesan Thirumalaivasan, Hirotsugu Hiramatsu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This paper reports the hyper-Raman (HR) spectra of the 20 amino acids in aqueous solution in the range of 400-1,800 cm(-1) with an excitation wavelength of 532 nm. A remarkable common feature in the nonresonance HR spectra is the large intensity of the HR bands of the COO- group. Whereas the peak position is mostly identical between the HR and Raman spectra, the intensity pattern is not. We discuss the similarities and dissimilarities between the pattern of the two spectra of each amino acid and give possible assignments to each HR band by comparing them with those of the corresponding Raman band. This study offers a reference for the HR spectra of the amino acids as the basic building block of proteins. It helps interpret the HR spectra of proteins and peptides.

Original languageEnglish
Number of pages14
JournalJournal of Raman Spectroscopy
DOIs
StateE-pub ahead of print - 20 Nov 2020

Keywords

  • amino acids
  • assignment
  • hyper&#8208
  • Raman
  • nonresonance
  • AMIDE-III VIBRATION
  • UV RESONANCE RAMAN
  • OPTICAL-ACTIVITY
  • SHORT PEPTIDES
  • SIDE-CHAINS
  • MOLECULAR-CONFORMATIONS
  • AQUEOUS-SOLUTIONS
  • INFRARED-SPECTRA
  • DFT CALCULATIONS
  • L-METHIONINE

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