3D pH measurement for cell culture using CT scan

Nobuya Higashino*, Toshio Takayama, Hiroaki Ito, Mitsuhiro Horade, Chia-Hung Tsai, Makoto Kaneko

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In cell culture, the pH of culture liquid is one of the most important conditions. However, culture liquid may have pH-gradient due to the activity of cells and change of the environment. In order to confirm the existence of pH-gradient, we propose a method for 3D pH measurement using the color change of phenol red. Using the principle of CT scan, we can measure pH-gradient without contacting culture liquid, and thus, decrease the possibility of contamination. In this paper, we consider the influence of light refraction by a cylindrical vessel, and show that we can measure local pH of culture liquid by light absorbance.

Original languageEnglish
Title of host publicationMHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1-3
Number of pages3
ISBN (Electronic)9781538633144
DOIs
StatePublished - 28 Feb 2018
Event28th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2017 - Nagoya, Japan
Duration: 3 Dec 20176 Dec 2017

Publication series

NameMHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science
Volume2018-January

Conference

Conference28th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2017
CountryJapan
CityNagoya
Period3/12/176/12/17

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