β -Lactoglobulin Influences Human Immunity and Promotes Cell Proliferation

Chun San Tai, Yi Yun Chen, Wen-Liang Chen*

*Corresponding author for this work

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

β-Lactoglobulin (LG) is suspected to enhance or modulate human immune responses. Moreover, LG is also hypothesized to increase human cell proliferation. However, these potential functions of LG have not been directly or thoroughly addressed. In this study, we demonstrated that LG is a potent stimulator of cell proliferation using a hybridoma cell (a splenocyte fused with a myeloma cell) model. LG's ability to promote cell proliferation was lost when the protein is denatured. To further investigate the influence of LG's conformation on cell proliferation, we chemically modified LG by either carboxymethylation (CM) or acetylation and observed significantly reduced cell proliferation when the protein structure was altered. Furthermore, we proved that LG enhances cell proliferation via receptor-mediated membrane IgM receptor. These data indicated that nondenatured LG is the major component in milk that modulates cell proliferation. Collectively, our study showed that LG plays a key role in enhancing immune responses by promoting cell proliferation through IgM receptor.

Original languageEnglish
Article number7123587
JournalBioMed Research International
Volume2016
DOIs
StatePublished - 1 Jan 2016

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